Translational_Unit

Part:BBa_K3704002:Design

Designed by: Kong Yangyang   Group: iGEM20_ECNUAS   (2020-10-17)


CMV-GR-SV40


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1305
    Illegal BamHI site found at 1458
    Illegal BamHI site found at 1572
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1870
    Illegal AgeI site found at 1158
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We mutated 744th. C to A to remove the SapI restriction site in Part: BBa_K3704000 to meet the type IIS standard.


Source

PubMed:27120390

References